Method for producing adeno-associated virus, cell, and expression vector

The present invention addresses the problem of providing a method for producing an adeno-associated virus capable of increasing the production amount of an adeno-associated virus, and also providing a cell and an expression vector used in the method for producing an adeno-associated virus. According to the present invention, provided is a method for producing an adeno-associated virus, said method comprising a step for expressing an E4ORF6 gene by means of an exogenous promoter, and wherein the expression of E4ORF6 is reduced compared to the expression of E4ORF6 using an expression unit comprising an enhancer, a cytomegalovirus-derived promoter, a Kozak sequence, an E4ORF6 gene, and a PolyA signal sequence. 本发明的课题在于提供一种能够提高腺相关病毒的产生量的腺相关病毒的制造方法，以及提供一种用于上述的腺相关病毒的制造方法中的细胞及表达载体。根据本发明，提供一种腺相关病毒的制造方法，其包括由外源启动子表达E4ORF6基因的步骤，其中，与使用包含增强子、来源于巨细胞病毒的启动子、Kozak序列、E4ORF6基因及PolyA信号序列的表达单元表达E4ORF6的情况相比，E4ORF6的表达降低。