Porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a
The invention particularly relates to a porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a, and belongs to the field of virus detection. Based on LAMP and CRISPR/Cas12a technologies, an SVA detection method i...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | WANG JING YANG JINCAI JIANG CHENGHUI LI XIAOMING LIU PING CHANG YANYAN GUO RONGXIA WANG HUIBAO YANG RUI |
| description | The invention particularly relates to a porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a, and belongs to the field of virus detection. Based on LAMP and CRISPR/Cas12a technologies, an SVA detection method is established, and a signal discrimination result is reported through a fluorescence method and a test strip method. The result of the embodiment shows that the method has strong specificity and high sensitivity. In addition, the method is convenient to operate, and the result is visualized; when the fluorescent reporter molecules exist in the system, the reaction is performed for 15 minutes at 15-37 DEG C, and the result can be observed through ultraviolet irradiation; when biotin reporter molecules exist in the system, reaction is carried out at 15-37 DEG C for 15 minutes, then test strip detection is carried out for 5 minutes, and the result can be observed by naked eyes. According to the method, the whole detec |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN118109649A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN118109649A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN118109649A3</originalsourceid><addsrcrecordid>eNqNzkEKwjAQBdBuXIh6h3Gni6JVEbssRVFQKbV7GZMpDbRJyKQewzMbxQO4mc-Hx2eG0aswTihNcCNNAuGpXM_hco8tSPIkvDIaOvKNkfBAJgmhl1V8zi4FzEp6kmOCyqFm4ZT98rMxNr6QVOiDP7HxDbkuLGadbVWtBH7YHFBLyMvTrSgXOXKywnE0qLFlmvxyFE0P-yo_xmTNndiiCG_6e35Nkl2yTLebNFv_Y969t0xR</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a</title><source>esp@cenet</source><creator>WANG JING ; YANG JINCAI ; JIANG CHENGHUI ; LI XIAOMING ; LIU PING ; CHANG YANYAN ; GUO RONGXIA ; WANG HUIBAO ; YANG RUI</creator><creatorcontrib>WANG JING ; YANG JINCAI ; JIANG CHENGHUI ; LI XIAOMING ; LIU PING ; CHANG YANYAN ; GUO RONGXIA ; WANG HUIBAO ; YANG RUI</creatorcontrib><description>The invention particularly relates to a porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a, and belongs to the field of virus detection. Based on LAMP and CRISPR/Cas12a technologies, an SVA detection method is established, and a signal discrimination result is reported through a fluorescence method and a test strip method. The result of the embodiment shows that the method has strong specificity and high sensitivity. In addition, the method is convenient to operate, and the result is visualized; when the fluorescent reporter molecules exist in the system, the reaction is performed for 15 minutes at 15-37 DEG C, and the result can be observed through ultraviolet irradiation; when biotin reporter molecules exist in the system, reaction is carried out at 15-37 DEG C for 15 minutes, then test strip detection is carried out for 5 minutes, and the result can be observed by naked eyes. According to the method, the whole detec</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240531&DB=EPODOC&CC=CN&NR=118109649A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240531&DB=EPODOC&CC=CN&NR=118109649A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG JING</creatorcontrib><creatorcontrib>YANG JINCAI</creatorcontrib><creatorcontrib>JIANG CHENGHUI</creatorcontrib><creatorcontrib>LI XIAOMING</creatorcontrib><creatorcontrib>LIU PING</creatorcontrib><creatorcontrib>CHANG YANYAN</creatorcontrib><creatorcontrib>GUO RONGXIA</creatorcontrib><creatorcontrib>WANG HUIBAO</creatorcontrib><creatorcontrib>YANG RUI</creatorcontrib><title>Porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a</title><description>The invention particularly relates to a porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a, and belongs to the field of virus detection. Based on LAMP and CRISPR/Cas12a technologies, an SVA detection method is established, and a signal discrimination result is reported through a fluorescence method and a test strip method. The result of the embodiment shows that the method has strong specificity and high sensitivity. In addition, the method is convenient to operate, and the result is visualized; when the fluorescent reporter molecules exist in the system, the reaction is performed for 15 minutes at 15-37 DEG C, and the result can be observed through ultraviolet irradiation; when biotin reporter molecules exist in the system, reaction is carried out at 15-37 DEG C for 15 minutes, then test strip detection is carried out for 5 minutes, and the result can be observed by naked eyes. According to the method, the whole detec</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzkEKwjAQBdBuXIh6h3Gni6JVEbssRVFQKbV7GZMpDbRJyKQewzMbxQO4mc-Hx2eG0aswTihNcCNNAuGpXM_hco8tSPIkvDIaOvKNkfBAJgmhl1V8zi4FzEp6kmOCyqFm4ZT98rMxNr6QVOiDP7HxDbkuLGadbVWtBH7YHFBLyMvTrSgXOXKywnE0qLFlmvxyFE0P-yo_xmTNndiiCG_6e35Nkl2yTLebNFv_Y969t0xR</recordid><startdate>20240531</startdate><enddate>20240531</enddate><creator>WANG JING</creator><creator>YANG JINCAI</creator><creator>JIANG CHENGHUI</creator><creator>LI XIAOMING</creator><creator>LIU PING</creator><creator>CHANG YANYAN</creator><creator>GUO RONGXIA</creator><creator>WANG HUIBAO</creator><creator>YANG RUI</creator><scope>EVB</scope></search><sort><creationdate>20240531</creationdate><title>Porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a</title><author>WANG JING ; YANG JINCAI ; JIANG CHENGHUI ; LI XIAOMING ; LIU PING ; CHANG YANYAN ; GUO RONGXIA ; WANG HUIBAO ; YANG RUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118109649A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG JING</creatorcontrib><creatorcontrib>YANG JINCAI</creatorcontrib><creatorcontrib>JIANG CHENGHUI</creatorcontrib><creatorcontrib>LI XIAOMING</creatorcontrib><creatorcontrib>LIU PING</creatorcontrib><creatorcontrib>CHANG YANYAN</creatorcontrib><creatorcontrib>GUO RONGXIA</creatorcontrib><creatorcontrib>WANG HUIBAO</creatorcontrib><creatorcontrib>YANG RUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG JING</au><au>YANG JINCAI</au><au>JIANG CHENGHUI</au><au>LI XIAOMING</au><au>LIU PING</au><au>CHANG YANYAN</au><au>GUO RONGXIA</au><au>WANG HUIBAO</au><au>YANG RUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a</title><date>2024-05-31</date><risdate>2024</risdate><abstract>The invention particularly relates to a porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a, and belongs to the field of virus detection. Based on LAMP and CRISPR/Cas12a technologies, an SVA detection method is established, and a signal discrimination result is reported through a fluorescence method and a test strip method. The result of the embodiment shows that the method has strong specificity and high sensitivity. In addition, the method is convenient to operate, and the result is visualized; when the fluorescent reporter molecules exist in the system, the reaction is performed for 15 minutes at 15-37 DEG C, and the result can be observed through ultraviolet irradiation; when biotin reporter molecules exist in the system, reaction is carried out at 15-37 DEG C for 15 minutes, then test strip detection is carried out for 5 minutes, and the result can be observed by naked eyes. According to the method, the whole detec</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN118109649A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T04%3A08%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=WANG%20JING&rft.date=2024-05-31&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN118109649A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |