Porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a

The invention particularly relates to a porcine Seneca virus visual detection method based on RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and CRISPR/Cas12a, and belongs to the field of virus detection. Based on LAMP and CRISPR/Cas12a technologies, an SVA detection method is established, and a signal discrimination result is reported through a fluorescence method and a test strip method. The result of the embodiment shows that the method has strong specificity and high sensitivity. In addition, the method is convenient to operate, and the result is visualized; when the fluorescent reporter molecules exist in the system, the reaction is performed for 15 minutes at 15-37 DEG C, and the result can be observed through ultraviolet irradiation; when biotin reporter molecules exist in the system, reaction is carried out at 15-37 DEG C for 15 minutes, then test strip detection is carried out for 5 minutes, and the result can be observed by naked eyes. According to the method, the whole detec