Method for rapidly breeding new variety of extra-early rice
The invention relates to the technical field of rice breeding, and discloses a method for rapidly breeding a new variety of very early rice, which comprises the following steps: carrying out a first round of PCR (Polymerase Chain Reaction) on DTH7, DTH8 and GHD7 in a to-be-detected rice population D...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to the technical field of rice breeding, and discloses a method for rapidly breeding a new variety of very early rice, which comprises the following steps: carrying out a first round of PCR (Polymerase Chain Reaction) on DTH7, DTH8 and GHD7 in a to-be-detected rice population DNA (Deoxyribonucleic Acid) extract by adopting an HI-TOM primer group I; a HI-TOM primer group II and a HI-TOM primer group III are adopted to carry out a second round of PCR on a product of the first round of PCR, and sequencing is carried out; according to the present invention, Hi-TOM is adopted to analyze the sequencing result, the existence conditions of the DTH7-8bp deelion9929 gene, the DTH8-1bp deelion323 gene and the GHD7-G/T157 gene in each rice plant in the rice population to be detected are determined, and the possible ultra-early rice variety is screened out; by adopting the method disclosed by the invention, the possible ultra-early rice variety can be accurately screened out, the range of subsequent |
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