Modeling method for promoting mouse obesity through SLC25A28 and application

The invention discloses a modeling method for promoting mouse obesity through SLC25A28 and application. The modeling method comprises the following steps: step 1, selection of experimental animals; step 2, construction of an adenovirus SLC25A28 vector and cell infection are carried out; step 3, perf...

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Hauptverfasser:	XIANG AOQI, GENG ZHANYI, WANG LE, GUAN HUA, YU QI, XING CHUNTAO, CHEN XIAOCHANG, DUAN BOWEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TESTING TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a modeling method for promoting mouse obesity through SLC25A28 and application. The modeling method comprises the following steps: step 1, selection of experimental animals; step 2, construction of an adenovirus SLC25A28 vector and cell infection are carried out; step 3, performing morphological analysis on the adipose tissue; and step 4, protein extraction and Western blotting analysis are carried out. According to the modeling method for promoting mouse obesity through the SLC25A28 and the application, the weight gain and lipid accumulation of the mouse can be promoted, and the mechanism shows that the expression of serum adiponectin protein is remarkably enhanced, and the expression level of serum FGF21 is remarkably reduced. 本发明公开的通过SLC25A28促进小鼠肥胖的建模方法及应用，包括以下步骤：步骤1、实验动物的选择；步骤2、腺病毒SLC25A28载体的构建及细胞感染；步骤3、脂肪组织形态学分析；步骤4、蛋白提取和Western blotting分析。本发明的通过SLC25A28促进小鼠肥胖的建模方法及应用，可以促进小鼠体重增加、脂质积累，机制发现血清脂联素蛋白表达显著增强，血清FGF21表达水平显著降低。