Transaminase and application thereof in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine
The invention provides a recombinant transaminase and an application of the recombinant transaminase in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine, and the transaminase is a mutant of a wild type transaminase. The transaminase mutant as shown in SEQ ID NO: 29, which is obtained by carrying...
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| creator | HU JICHENG ZHANG YUE WANG SONGHE YUE YONGTANG |
| description | The invention provides a recombinant transaminase and an application of the recombinant transaminase in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine, and the transaminase is a mutant of a wild type transaminase. The transaminase mutant as shown in SEQ ID NO: 29, which is obtained by carrying out substitution mutation on one or more amino acids in 13th-312th amino acids of wild type transaminase as shown in SEQ ID NO: 1, has remarkably improved transaminase activity, can efficiently catalyze 4-chloro-1-(2, 5-difluorophenyl) butyl-1-one to synthesize (R)-2-(2, 5-difluorophenyl) pyrrolidine, is mild in reaction conditions, is high in yield, and can be used for preparing the (R)-2-(2, 5-difluorophenyl) pyrrolidine. The preparation method is simple, the cost is low, and the preparation method has important significance in the field of industrial production of (R)-2-(2, 5-difluorophenyl) pyrrolidine and lalotinib.
本发明提供了重组转氨酶及其在合成(R)-2-(2,5-二氟苯基)吡咯烷中的应用，所述转氨酶是野生型转氨酶的突变体。本发明通过对SEQ ID NO:1所示的野生型转氨酶的第13～312位氨基 |
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本发明提供了重组转氨酶及其在合成(R)-2-(2,5-二氟苯基)吡咯烷中的应用，所述转氨酶是野生型转氨酶的突变体。本发明通过对SEQ ID NO:1所示的野生型转氨酶的第13～312位氨基</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240531&DB=EPODOC&CC=CN&NR=118109434A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240531&DB=EPODOC&CC=CN&NR=118109434A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HU JICHENG</creatorcontrib><creatorcontrib>ZHANG YUE</creatorcontrib><creatorcontrib>WANG SONGHE</creatorcontrib><creatorcontrib>YUE YONGTANG</creatorcontrib><title>Transaminase and application thereof in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine</title><description>The invention provides a recombinant transaminase and an application of the recombinant transaminase in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine, and the transaminase is a mutant of a wild type transaminase. The transaminase mutant as shown in SEQ ID NO: 29, which is obtained by carrying out substitution mutation on one or more amino acids in 13th-312th amino acids of wild type transaminase as shown in SEQ ID NO: 1, has remarkably improved transaminase activity, can efficiently catalyze 4-chloro-1-(2, 5-difluorophenyl) butyl-1-one to synthesize (R)-2-(2, 5-difluorophenyl) pyrrolidine, is mild in reaction conditions, is high in yield, and can be used for preparing the (R)-2-(2, 5-difluorophenyl) pyrrolidine. The preparation method is simple, the cost is low, and the preparation method has important significance in the field of industrial production of (R)-2-(2, 5-difluorophenyl) pyrrolidine and lalotinib.
本发明提供了重组转氨酶及其在合成(R)-2-(2,5-二氟苯基)吡咯烷中的应用，所述转氨酶是野生型转氨酶的突变体。本发明通过对SEQ ID NO:1所示的野生型转氨酶的第13～312位氨基</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyj0KwkAQQOE0FqLeYdIl4IL5EbSUoFhZSEohDNkJGVhnl51Y5Pam8ABWjw_eOnm1EUXxzYJKgGIBQ3Dc48ReYBopkh-ABXSWRcoKi7NnbkqTlXs4GsuD-_jow0gyuxzCHKN3bFlom6wGdEq7XzdJeru2zd1Q8B1pwJ6Epq55FMWpOJzrqr5U_zxfD7k6dg</recordid><startdate>20240531</startdate><enddate>20240531</enddate><creator>HU JICHENG</creator><creator>ZHANG YUE</creator><creator>WANG SONGHE</creator><creator>YUE YONGTANG</creator><scope>EVB</scope></search><sort><creationdate>20240531</creationdate><title>Transaminase and application thereof in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine</title><author>HU JICHENG ; ZHANG YUE ; WANG SONGHE ; YUE YONGTANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118109434A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HU JICHENG</creatorcontrib><creatorcontrib>ZHANG YUE</creatorcontrib><creatorcontrib>WANG SONGHE</creatorcontrib><creatorcontrib>YUE YONGTANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HU JICHENG</au><au>ZHANG YUE</au><au>WANG SONGHE</au><au>YUE YONGTANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Transaminase and application thereof in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine</title><date>2024-05-31</date><risdate>2024</risdate><abstract>The invention provides a recombinant transaminase and an application of the recombinant transaminase in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine, and the transaminase is a mutant of a wild type transaminase. The transaminase mutant as shown in SEQ ID NO: 29, which is obtained by carrying out substitution mutation on one or more amino acids in 13th-312th amino acids of wild type transaminase as shown in SEQ ID NO: 1, has remarkably improved transaminase activity, can efficiently catalyze 4-chloro-1-(2, 5-difluorophenyl) butyl-1-one to synthesize (R)-2-(2, 5-difluorophenyl) pyrrolidine, is mild in reaction conditions, is high in yield, and can be used for preparing the (R)-2-(2, 5-difluorophenyl) pyrrolidine. The preparation method is simple, the cost is low, and the preparation method has important significance in the field of industrial production of (R)-2-(2, 5-difluorophenyl) pyrrolidine and lalotinib.
本发明提供了重组转氨酶及其在合成(R)-2-(2,5-二氟苯基)吡咯烷中的应用，所述转氨酶是野生型转氨酶的突变体。本发明通过对SEQ ID NO:1所示的野生型转氨酶的第13～312位氨基</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Transaminase and application thereof in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine |
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