Transaminase and application thereof in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine

The invention provides a recombinant transaminase and an application of the recombinant transaminase in synthesis of (R)-2-(2, 5-difluorophenyl) pyrrolidine, and the transaminase is a mutant of a wild type transaminase. The transaminase mutant as shown in SEQ ID NO: 29, which is obtained by carrying out substitution mutation on one or more amino acids in 13th-312th amino acids of wild type transaminase as shown in SEQ ID NO: 1, has remarkably improved transaminase activity, can efficiently catalyze 4-chloro-1-(2, 5-difluorophenyl) butyl-1-one to synthesize (R)-2-(2, 5-difluorophenyl) pyrrolidine, is mild in reaction conditions, is high in yield, and can be used for preparing the (R)-2-(2, 5-difluorophenyl) pyrrolidine. The preparation method is simple, the cost is low, and the preparation method has important significance in the field of industrial production of (R)-2-(2, 5-difluorophenyl) pyrrolidine and lalotinib. 本发明提供了重组转氨酶及其在合成(R)-2-(2,5-二氟苯基)吡咯烷中的应用，所述转氨酶是野生型转氨酶的突变体。本发明通过对SEQ ID NO:1所示的野生型转氨酶的第13～312位氨基