Phycomycete mixed preparation, preparation method thereof and application of phycomycete mixed preparation in soil improvement
The invention provides a phycomycete mixed preparation as well as a preparation method and application thereof in soil improvement, and belongs to the technical field of soil improvement. The method disclosed by the invention comprises the following steps: 1, inoculating algae into a liquid culture...
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| creator | TUHERTY, ISAJIANG ABOUDOUAINE, KAYLA ABULIKM, AKBAR LIANG CHENGCHEN |
| description | The invention provides a phycomycete mixed preparation as well as a preparation method and application thereof in soil improvement, and belongs to the technical field of soil improvement. The method disclosed by the invention comprises the following steps: 1, inoculating algae into a liquid culture medium for culturing, and measuring biomass and microscope cell count at regular time every day; 2, after the algae cells enter a stationary phase, taking a proper amount of algae cells, centrifuging, respectively collecting supernate and algae mud, and crushing an algae concentrated solution; 3, activating and culturing bacillus subtilis, and centrifuging to obtain a bacterial suspension when the bacillus subtilis enters a logarithmic phase; 4, inoculating the prepared algae supernatant, algae concentrated solution and algae crushed solution into the bacterial suspension for continuous culture; and 5, culturing for 16 hours, spraying to the surface of the soil, and carrying out daily maintenance. By means of the m |
| format | Patent |
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The method disclosed by the invention comprises the following steps: 1, inoculating algae into a liquid culture medium for culturing, and measuring biomass and microscope cell count at regular time every day; 2, after the algae cells enter a stationary phase, taking a proper amount of algae cells, centrifuging, respectively collecting supernate and algae mud, and crushing an algae concentrated solution; 3, activating and culturing bacillus subtilis, and centrifuging to obtain a bacterial suspension when the bacillus subtilis enters a logarithmic phase; 4, inoculating the prepared algae supernatant, algae concentrated solution and algae crushed solution into the bacterial suspension for continuous culture; and 5, culturing for 16 hours, spraying to the surface of the soil, and carrying out daily maintenance. 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| subjects | ADHESIVES BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DYES ENZYMOLOGY MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS PAINTS POLISHES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Phycomycete mixed preparation, preparation method thereof and application of phycomycete mixed preparation in soil improvement |
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