Phycomycete mixed preparation, preparation method thereof and application of phycomycete mixed preparation in soil improvement

The invention provides a phycomycete mixed preparation as well as a preparation method and application thereof in soil improvement, and belongs to the technical field of soil improvement. The method disclosed by the invention comprises the following steps: 1, inoculating algae into a liquid culture...

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Hauptverfasser: TUHERTY, ISAJIANG, ABOUDOUAINE, KAYLA, ABULIKM, AKBAR, LIANG CHENGCHEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a phycomycete mixed preparation as well as a preparation method and application thereof in soil improvement, and belongs to the technical field of soil improvement. The method disclosed by the invention comprises the following steps: 1, inoculating algae into a liquid culture medium for culturing, and measuring biomass and microscope cell count at regular time every day; 2, after the algae cells enter a stationary phase, taking a proper amount of algae cells, centrifuging, respectively collecting supernate and algae mud, and crushing an algae concentrated solution; 3, activating and culturing bacillus subtilis, and centrifuging to obtain a bacterial suspension when the bacillus subtilis enters a logarithmic phase; 4, inoculating the prepared algae supernatant, algae concentrated solution and algae crushed solution into the bacterial suspension for continuous culture; and 5, culturing for 16 hours, spraying to the surface of the soil, and carrying out daily maintenance. By means of the m