High-throughput nucleic acid synthesis chip and use method thereof

The chip for nucleic acid synthesis comprises a plurality of synthesis units, each synthesis unit comprises a plurality of three-dimensional layers, the first layer in the plurality of three-dimensional layers is a synthesis layer of short nucleic acid sequences, and the second layer to the last layer are splicing layers of nucleic acid sequences. Wherein a plurality of synthesis reaction points exist in the first layer to synthesize a plurality of short nucleic acid sequences, and splicing reaction points exist from the second layer to the last layer to sequentially splice the short nucleic acid sequences synthesized in the previous layer into long nucleic acid sequences until the nucleic acid sequence with complete length is generated. 一种用于核酸合成的芯片，所述芯片包含多个合成单元，每个合成单元包含多个立体层，其中所述多个立体层中的第一层为短核酸序列的合成层，第二层至最后一层为核酸序列的拼接层，其中在第一层存在多个合成反应点以合成多个短核酸序列，在第二层至最后一层存在拼接反应点以将上一层合成的较短核酸序列有序拼接为较长核酸序列，直至产生完整长度的核酸序列。