Human chorionic gonadotropin detection kit and preparation method thereof

The invention provides a human chorionic gonadotropin detection kit and a preparation method thereof. The kit comprises a plastic backboard, a card shell, a sample pad, a latex microsphere pad, a nitrocellulose membrane and composite absorbent paper, and the sample pad comprises a glass fiber membrane and a sample pad treating fluid; the latex microsphere pad comprises a glass fiber membrane and modified latex microspheres marked with a mouse anti-beta-HCG monoclonal antibody; a detection line and a quality control line are marked on the surface of the nitrocellulose membrane according to the flowing direction of a sample, the detection line is coated with a mouse anti-alpha-HCG monoclonal antibody, and the quality control line is coated with a goat anti-mouse IgG polyclonal antibody; the sample pad treating fluid is prepared from the following raw materials in percentage by weight: 2 to 7 percent of Na2HPO4. 12H2O, 0.2 to 0.3 percent of chitosan oligosaccharide, 0.5 to 2.5 percent of polyethylene glycol, 0.5