Method for detecting impurity amino acids in L-valine

The invention discloses a method for detecting impurity amino acids in L-valine, which adopts a silica gel-based C18 reversed-phase chromatographic column, a specific mobile phase and an elution procedure, separates and detects impurity amino acid residues in L-valine through high performance liquid chromatography, and has the advantages of high detection signal-to-noise ratio, high theoretical plate number and low quantitation limit. The detection requirement that the single impurity amino acid residue in the high-purity L-valine is less than or equal to 0.02% is met. 本发明公开了一种L-缬氨酸中杂质氨基酸的检测方法，采用硅胶基C18反相色谱柱及特定流动相和洗脱程序，通过高效液相色谱法分离检测L-缬氨酸中杂质氨基酸残留，检测信噪比高，理论塔板数高，定量限低，满足对高纯L-缬氨酸中单个杂质氨基酸残留要求≤0.02％的检测要求。