SNP (Single Nucleotide Polymorphism) site combination, primer combination and method for identifying breeds of WuMongolian black-bone chickens

The invention discloses an SNP (Single Nucleotide Polymorphism) site combination, a primer combination and a method for identifying breeds of WMWG6 silky fowl. The SNP site combination comprises a WMWG1 site to a WMWG6 site. When the SNP site combination provided by the invention is used for identif...

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Hauptverfasser: LUO KEYIN, LIU YIFAN, TU YUNJIE, SHENG ZHONGWEI, ZOU JIANMIN, WANG XIANKE, JU XIAOJUN, JI GAIGE, SHU JINGTING, LI CHUNMIAO, ZHANG MING, WANG GUANJI, SHAN YANJU, XIAO QIN
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creator LUO KEYIN
LIU YIFAN
TU YUNJIE
SHENG ZHONGWEI
ZOU JIANMIN
WANG XIANKE
JU XIAOJUN
JI GAIGE
SHU JINGTING
LI CHUNMIAO
ZHANG MING
WANG GUANJI
SHAN YANJU
XIAO QIN
description The invention discloses an SNP (Single Nucleotide Polymorphism) site combination, a primer combination and a method for identifying breeds of WMWG6 silky fowl. The SNP site combination comprises a WMWG1 site to a WMWG6 site. When the SNP site combination provided by the invention is used for identifying the variety of the UMongolia black-bone chicken, the probability that all six SNP site combined genotypes are judged as the UMongolia black-bone chicken is 100%, and as long as one SNP site genotype does not conform to the corresponding genotype of the UMongolia black-bone chicken, the individual can be completely excluded from belonging to the UMongolia black-bone chicken. The method is simple and convenient to operate and high in accuracy, and can effectively strike behaviors of fake UMongolia black-bone chicken products in the market. 本发明公开了一种用于乌蒙乌骨鸡品种鉴定的SNP位点组合、引物组合和方法,所述SNP位点组合包括WMWG1位点到WMWG6位点。采用本发明提供的SNP位点组合进行乌蒙乌骨鸡品种鉴定时,全部6个SNP位点联合基因型判定为乌蒙乌骨鸡的概率为100%,只要有一个SNP位点基因型不符合乌蒙乌骨鸡相应的基因型,即可完全排除该个体属于乌蒙乌骨鸡。这种方法操作简便
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title SNP (Single Nucleotide Polymorphism) site combination, primer combination and method for identifying breeds of WuMongolian black-bone chickens
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