SNP (Single Nucleotide Polymorphism) site combination, primer combination and method for identifying breeds of WuMongolian black-bone chickens

The invention discloses an SNP (Single Nucleotide Polymorphism) site combination, a primer combination and a method for identifying breeds of WMWG6 silky fowl. The SNP site combination comprises a WMWG1 site to a WMWG6 site. When the SNP site combination provided by the invention is used for identifying the variety of the UMongolia black-bone chicken, the probability that all six SNP site combined genotypes are judged as the UMongolia black-bone chicken is 100%, and as long as one SNP site genotype does not conform to the corresponding genotype of the UMongolia black-bone chicken, the individual can be completely excluded from belonging to the UMongolia black-bone chicken. The method is simple and convenient to operate and high in accuracy, and can effectively strike behaviors of fake UMongolia black-bone chicken products in the market. 本发明公开了一种用于乌蒙乌骨鸡品种鉴定的SNP位点组合、引物组合和方法，所述SNP位点组合包括WMWG1位点到WMWG6位点。采用本发明提供的SNP位点组合进行乌蒙乌骨鸡品种鉴定时，全部6个SNP位点联合基因型判定为乌蒙乌骨鸡的概率为100％，只要有一个SNP位点基因型不符合乌蒙乌骨鸡相应的基因型，即可完全排除该个体属于乌蒙乌骨鸡。这种方法操作简便