Application of glutamate dehydrogenase and glutamic-pyruvic transaminase circular reaction system in method for detecting enzyme activity and enzyme substrate

The invention belongs to the technical field of substance detection, and particularly relates to application of a glutamate dehydrogenase and glutamic-pyruvic transaminase circular reaction system in a method for detecting enzyme activity and an enzyme substrate. According to the invention, a glutamate dehydrogenase and glutamic-pyruvic transaminase circular reaction system is applied to the detection process of enzyme activity and enzyme substrates for the first time, and the detection method is used for determining the activity of kinase and transaminase and determining corresponding substrates according to the principle of double-enzyme circular catalytic reaction determination. According to the invention, detection of fructose and pyruvic acid can be realized based on two circular reaction systems, high specificity, high sensitivity and wide detection range of fructose detection are realized through a principle of determination by a double-cycle enzyme kinetics method, and a new means is provided for kina