Excrement nucleic acid extraction or purification reagent and preparation method thereof

The invention relates to a reagent, in particular to an excrement nucleic acid extraction or purification reagent and a preparation method thereof. The reagent comprises the following components: a lysis solution, a binding solution, magnetic beads, a washing solution 1, a washing solution 2, an elu...

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Hauptverfasser: SHI CONG, WANG JUNHONG, CHEN QINYU, JIANG JIANZHONG, JIN CHUNYU, JIANG QINGXIA
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to a reagent, in particular to an excrement nucleic acid extraction or purification reagent and a preparation method thereof. The reagent comprises the following components: a lysis solution, a binding solution, magnetic beads, a washing solution 1, a washing solution 2, an eluent and protease K, the method comprises the following operation steps: sample collection, cracking, combination, washing, drying and elution. The method has the advantages of few components, simple operation process and normal temperature preservation of the reagent, and the extracted product can be used for subsequent experiments such as PCR amplification and methylation. 本发明涉及一种试剂,尤其涉及一种粪便核酸提取或纯化试剂及制备方法。试剂包括以下成分:裂解液、结合液、磁珠、洗涤液1、洗涤液2、洗脱液、蛋白酶K;通过以下操作步骤:样本采集→裂解→结合→洗涤→干燥→洗脱。组分较少、操作流程简单、试剂可常温保存,提取的产物可用于PCR扩增、甲基化等后续实验。