Rhamnolipid acyltransferase mutant as well as preparation method and application thereof

The invention provides a rhamnolipid acyltransferase mutant as well as a preparation method and application thereof. Compared with the amino acid sequence of the wild type rhamnolipid acyltransferase, the mutant of the rhamnolipid acyltransferase disclosed by the invention has the mutation of one or more sites as follows: M37, D201, A142, A101, A182, F43, V123, H216, S42, L61, R74, Q133, Q143, Q146, E147, L148, S173, N174, H177, M178, H192, Q195, L208, W222, R232, Q73, E209, Q176, Q212, P72, G184 and E187. Escherichia coli capable of producing the mutant disclosed by the invention is constructed, and when the escherichia coli is used for producing rhamnolipid, the Rha-C10-C8 configuration content is obviously different from that of a wild type. 本发明提供了一种鼠李糖脂酰基转移酶突变体及其制备方法与应用。本发明的鼠李糖脂酰基转移酶的突变体，相比于野生型鼠李糖脂酰基转移酶的氨基酸序列具有以下一个或多个位点的突变：M37、D201、A142、A101、A182、F43、V123、H216、S42、L61、R74、Q133、Q143、Q146、E147、L148、S173、N174、H177、M178、H192、Q195、L208、W222、R232、Q73、E209、Q176、Q212、P72、G184、E187。构建可产生本发明的突变体的大肠杆菌，用于生产鼠李糖脂时，Rha-