Method for culturing cryopreserved umbilical cord blood NK (Natural Killer) cells

The invention provides a culture method of cryopreserved umbilical cord blood NK cells, and relates to the technical field of biology. According to the culture method of the cryopreserved umbilical cord blood NK cells, the cryopreservation liquid of the recovered umbilical cord blood is removed, then activation and amplification of the NK cells are directly carried out, a conventional mononuclear cell separation process is not carried out, the operation process is simplified, the recovery rate of the mononuclear cells and the NK cells of the cryopreserved umbilical cord blood is increased, and the culture cost is reduced. The in-vitro amplification capability and killing activity of the NK cells are not influenced, so that the final yield of the in-vitro amplified NK cells of one part of cryopreserved umbilical cord blood can be obviously improved. According to the method, a small molecule combination CEPT (Chroman 1, Emricasan, Trans-ISRIB and Polyamine Solution) is added into a culture medium for activated