Complete culture medium freeze-dried powder and application thereof

The invention belongs to the technical field of biological medicine, and particularly discloses complete culture medium freeze-dried powder and application thereof. The culture medium is characterized in that the following components are added into a basic culture medium: 4-hydroxyethyl piperazine ethanesulfonic acid, vitamin C, vitamin E, an epidermal growth factor, a basic fibroblast growth factor, fetal calf serum, sodium selenite, sodium ascorbate phosphate, transferrin, glucose, fetal calf serum, proline, asparagine, magnesium sulfate and a freeze-drying protective agent. By adding the freeze-drying protective agent, the complete culture medium can be effectively protected and prevented from being polluted, the stability of the complete culture medium is improved, and the activity and the stability of the complete culture medium can be effectively maintained, so that cells can be effectively amplified during use, and the cell viability is high. 本发明属于生物医药技术领域，本发明具体公开了一种完全型培养基冻干粉及其应用，在基础培养基中添加以下组分：4-羟乙基哌嗪乙