Recombinant host cell for producing malonic acid

The present invention relates to methods for biologically producing malonic acid; screening for malonic acid producing host cells having improved malonic acid production; purifying the biologically produced malonic acid; and recombinant host cells, materials and methods for converting malonic acid synthesis into industrially important chemicals. The recombinant host cell provided by the invention is a yeast cell or a bacterial cell, and comprises a heterologous malonyl-coenzyme A hydrolase. The recombinant host cells of the present invention comprise genetic modifications that increase acetyl-coenzyme A biosynthesis, increase malonyl-coenzyme A biosynthesis, reduce malonic acid catabolism, increase malonic acid secretion from the host cell, increase host cell tolerance to malonic acid, and increase catabolism of various carbon sources, thereby increasing malonic acid yield, titer, and/or productivity. 本发明涉及用于生物产生丙二酸；筛选丙二酸产生得以改进的丙二酸产生性宿主细胞；纯化生物产生的丙二酸；以及使丙二酸合成转化成工业重要化学品的重组宿主细胞、材料和方法。本发明的重组宿主细胞，其为酵母细胞或细菌细胞，其包含异源