Preparation method of HbA1c monoclonal antibody for chromatography and turbidimetry double platforms
The invention discloses a preparation method of an HbA1c monoclonal antibody for chromatography and turbidimetry double platforms, and belongs to the technical field of biology, and the preparation method comprises the following steps: S1, antigen preparation; s2, animal immunization; s3, carrying o...
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| creator | ZHANG ZHIPENG ZHANG LEI YU CHUNYUN MIAO LIANJUN |
| description | The invention discloses a preparation method of an HbA1c monoclonal antibody for chromatography and turbidimetry double platforms, and belongs to the technical field of biology, and the preparation method comprises the following steps: S1, antigen preparation; s2, animal immunization; s3, carrying out cell fusion; s4, cloning, screening and hybridoma strain establishment; s5, ascites preparation and antibody purification; s6, comparing and evaluating the HbA1c antibody obtained in the step S5 with phenpeng and Eurja HbA1C antibodies on a turbidimetric platform, observing the suitability of the HbA1c antibody and the phenpeng and Eurja HbA1C antibodies with goat anti-mouse I gG, performing immunochromatography test on the Hb and HbA1c antibodies obtained in the step S5, and observing the sensitivity of the HbA1c antibody; according to the invention, the monoclonal antibody correspondingly cloned by Hb and the monoclonal antibody correspondingly cloned by HbA1c are successfully obtained, and the HbAl c antibody |
| format | Patent |
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s2, animal immunization; s3, carrying out cell fusion; s4, cloning, screening and hybridoma strain establishment; s5, ascites preparation and antibody purification; s6, comparing and evaluating the HbA1c antibody obtained in the step S5 with phenpeng and Eurja HbA1C antibodies on a turbidimetric platform, observing the suitability of the HbA1c antibody and the phenpeng and Eurja HbA1C antibodies with goat anti-mouse I gG, performing immunochromatography test on the Hb and HbA1c antibodies obtained in the step S5, and observing the sensitivity of the HbA1c antibody; according to the invention, the monoclonal antibody correspondingly cloned by Hb and the monoclonal antibody correspondingly cloned by HbA1c are successfully obtained, and the HbAl c antibody</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Preparation method of HbA1c monoclonal antibody for chromatography and turbidimetry double platforms |
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