Preparation method of HbA1c monoclonal antibody for chromatography and turbidimetry double platforms

The invention discloses a preparation method of an HbA1c monoclonal antibody for chromatography and turbidimetry double platforms, and belongs to the technical field of biology, and the preparation method comprises the following steps: S1, antigen preparation; s2, animal immunization; s3, carrying out cell fusion; s4, cloning, screening and hybridoma strain establishment; s5, ascites preparation and antibody purification; s6, comparing and evaluating the HbA1c antibody obtained in the step S5 with phenpeng and Eurja HbA1C antibodies on a turbidimetric platform, observing the suitability of the HbA1c antibody and the phenpeng and Eurja HbA1C antibodies with goat anti-mouse I gG, performing immunochromatography test on the Hb and HbA1c antibodies obtained in the step S5, and observing the sensitivity of the HbA1c antibody; according to the invention, the monoclonal antibody correspondingly cloned by Hb and the monoclonal antibody correspondingly cloned by HbA1c are successfully obtained, and the HbAl c antibody