Preparation method of clinical-grade neural stem cell cryopreservation preparation

The invention discloses a preparation method of a clinical-grade neural stem cell cryopreservation preparation. The preparation method comprises the following steps: carrying out suspension multiplication culture on neural stem cells; adherent multiplication culture of the neural stem cells; neural stem cell single cell culture and stock solution preparation: preparing a cryopreservation solution suspension cell stock solution, and performing cryopreservation. According to the technical scheme, in the suspension amplification stage, multiplication culture is carried out in a low-adsorption culture bottle neural sphere mode, and differentiation and stem cell attribute reduction caused by neural sphere attachment are avoided; in the adherent amplification stage, matrigel is adopted for 3D single cell amplification culture, the attribute of stem cells is maintained, and cell proliferation is not delayed; the preparation stock solution is prepared after the single cells are subjected to suspension culture for 24