Tissue culture method of pennisetum alopecuroides

The invention provides a tissue culture method of pennisetum alopecuroides, and relates to the technical field of plant tissue culture. The tissue culture method of the pennisetum alopecuroides comprises the following steps: disinfecting explants, adding a proper amount of IBA and 2, 4-D into a culture medium to perform callus induction, inducing cluster buds by adopting a culture medium added with a proper amount of IBA and BA, and finally performing rooting culture by adopting a culture medium added with IBA, NAA, aspirin and 25% propyl gallate aqueous solution. And domesticating and transplanting the obtained rooted Chinese pennisetum. According to the method, the defects in the prior art are overcome, the rooted pennisetum alopecuroides seedlings are cultured through the tissue culture technology, follow-up planting and breeding are facilitated, the rooting rate and the root system quality of tissue culture are improved by adjusting the composition of the culture medium, and follow-up planting survival is