Method for efficiently breeding saxifraga stolonifera through stolons

The invention provides a method for efficiently breeding saxifraga stolonifera through stolons, which at least comprises the following steps: taking saxifraga stolonifera stolons with bud points as explants, and sequentially carrying out culture treatment including callus induction culture and adventitious bud induction culture on the explants to obtain saxifraga stolonifera transplanted seedlings; wherein an induction culture medium used in the callus induction culture comprises one of MS + ZT + 2, 4-D + NAA, MS + 2, 4-D + NAA, MS + ZT + NAA, MS + ZT + KT + 2, 4-D + NAA; an adventitious bud induction culture medium used in the adventitious bud induction culture comprises one of MS + ZT + TDZ + IBA, MS + TDZ + IBA, MS + ZT + IBA and MS + ZT + TDZ + IBA + GA3. The method does not damage the saxifraga stolonifera female parent, and is short in breeding cycle and high in hereditary stability. 本发明提供一种通过匍匐茎高效繁育虎耳草的方法，至少包括以下步骤：采用具有芽点的虎耳草匍匐茎作为外植体，对所述外植体依次进行包括愈伤组织诱导培养、不定芽诱导培养的培养处理，得到虎耳草移栽苗；其中所述愈伤组织诱导培养使用的诱导培养基包括MS+ZT