Preparation method of four-unit antigen for hemagglutination inhibition test

The invention relates to the technical field of pathogen or antibody detection by using hemagglutination inhibition, in particular to a four-unit antigen preparation method for a hemagglutination inhibition test. The four-unit antigen preparation method for the hemagglutination inhibition test aims at solving the technical problems that in the prior art, repeated calibration and adjustment are needed, an adjustment method after inaccurate detection is not defined, and the calculation difficulty is large. According to the technical scheme, the preparation method of the four-unit antigen for the hemagglutination inhibition test comprises the following steps: step 1, diluting; 2, adding PBS (Phosphate Buffer Solution); step 3, adding a to-be-used antigen or a diluent of the to-be-used antigen; step 4, fully and uniformly mixing; step 5, supplementing the PBS; 6, adding the chicken red blood cell suspension; 7, recording the maximum column number and the antigen hemagglutination titer; step 8, selecting a maximum