Pseudosciaena crocea megalocytovirus typing detection primer, kit and application thereof

The invention relates to a larimichthys crocea megalocytovirus typing detection primer, a kit and application thereof. The large yellow croaker megalocytovirus detection primer is designed and obtained, and the large yellow croaker megalocytovirus is detected through a specific method amplification system and conditions based on the primer. The PCR detection primer disclosed by the invention is high in specificity and sensitivity, and can be used for identifying whether the larimichthys crocea tissue contains the megalocytivirus or not. The kit can be used for detecting the typing of the larimichthys crocea swollen cell virus, provides early diagnosis and genetic typing for the larimichthys crocea iridovirus disease, and provides effective technical support for molecular epidemiological investigation of the larimichthys crocea iridovirus disease. 本发明涉及一种大黄鱼肿大细胞病毒分型检测引物、试剂盒及其应用。本发明设计得到大黄鱼肿大细胞病毒检测引物，并基于该引物，通过特定法扩增体系和条件检测大黄鱼肿大细胞病毒。本发明的PCR检测引物特异性强，灵敏度高，能够从大黄鱼组织中鉴别是否含有肿大细胞病毒。本发明可以用于大黄鱼肿大细胞病毒的分型的检测，为大黄鱼虹彩病毒病提供早期诊断、