Taqman probe for detecting mycobacterium population and method and application thereof

The invention discloses a Taqman probe for detecting a mycobacterium abscessus, mycobacterium tuberculosis and mycobacterium avium complex group, which is characterized in that a target probe and a primer with specific site difference are designed by taking an hsp65 gene conserved region of a strain as a target spot, and the probe and the primer are used for carrying out fluorescent quantitative PCR (Polymerase Chain Reaction) to realize specific identification of the strain. By designing the specific probes and primers, the mycobacterium abscessus, mycobacterium tuberculosis and mycobacterium avium complex group can be rapidly and accurately detected, high sensitivity and specificity are achieved, operation is easy, convenient and rapid, an accurate detection result can be obtained within a short time, and wide application prospects are achieved. 本发明公开了一种检测脓肿分枝杆菌、结核分枝杆菌、鸟分枝杆菌复合群的Taqman探针，其以菌株的hsp65基因保守区域为靶点，设计具有特异性位点差异的靶向探针和引物，用探针和引物进行荧光定量PCR以实现菌种的特异性鉴定。本发明通过设计特异性的探针和引物，能够快速、准确地检测脓肿分枝杆菌、结核分枝杆菌、鸟分枝杆菌复合群，具有高度的