Method for detecting action mechanism of miR-23b-27b-24-1 cluster in chronic kidney disease

The invention relates to the technical field of biological medicines, in particular to a method for detecting an action mechanism of a miR-23b-27b-24-1 cluster in chronic kidney diseases, which comprises the following steps: determining a relationship between expression of the miR-23b-27b-24-1 cluster and renal tubular EMT and renal fibrosis caused by chronic hypoxia; the upstream mechanism of the miR-23b-27b-24-1 cluster regulated and expressed by the HIF-1alpha is determined; and determining that the miR-23b-27b-24-1 cluster participates in renal tubular epithelial cell transdifferentiation caused by hypoxia and a downstream molecular mechanism. The key signal channel of the HIF-1alpha-miR-23b-27b-24-1 cluster is elaborated for the first time, and the effect of the HIF-1alpha-miR-23b-27b-24-1 cluster in transdifferentiation and fibrosis of chronic nephropathy is deeply researched, so that a brand new perspective is provided for kidney transdifferentiation and fibrosis caused by chronic hypoxia, and an exper