Heavy metal ion detection kit based on double CRISPR-Cas system, detection method and application

The invention belongs to the technical field of heavy metal detection, and particularly relates to a heavy metal ion detection kit based on a double CRISPR-Cas system, a detection method and application. The double-stranded DNA in the DNAzyme probe of the heavy metal ion detection kit provided by the invention can activate Cas12a to cut the I/H1/H2 probe to generate a large number of repeated H1/H2 double-stranded DNA, the H1/H2 double-stranded DNA can activate the Cas12a to cut the labeled fluorescence/quenched single-stranded DNA to generate a fluorescence signal, and by improving the I/H1/H2 probe and the double CRISPR-Cas system in the heavy metal ion detection kit, the detection sensitivity of the heavy metal ion detection kit is improved. The technical problem that when an existing heavy metal ion detection kit is used for detecting heavy metal ions, a CRISPR-Cas system needs to be subjected to pre-DNA amplification is solved. 本申请属于重金属检测技术领域，尤其涉及一种基于双重CRISPR-Cas系统的重金属离子检测试剂盒、检测方法和应用；本申请提供的重金属离子检测试剂盒的DNA