Several polyphosphate kinases capable of efficiently regenerating GTP and application of polyphosphate kinases

The invention discloses a plurality of polyphosphate kinases (PPK) capable of efficiently regenerating GTP and application of the polyphosphate kinases, and belongs to the technical field of biology. According to the invention, polyphosphate kinase expressed and obtained in Escherichia coli BL21 (DE3) can efficiently regenerate GTP by taking tripolyphosphate, tetrapolyphosphate, hexametaphosphate and the like as phosphoric acid donors. When the PPK is used for GTP regeneration and synthesis of GDP-L-fucose or GDP-mannose and derivative products thereof (such as fucosyllactose), the sustainability of the reaction is remarkably improved, and the reaction cost is reduced. 本发明公开了几种高效再生GTP的多聚磷酸激酶(PPK)及其应用，属于生物技术领域。本发明在大肠杆菌BL21(DE3)中表达并获得的多聚磷酸盐激酶，能以三聚磷酸盐、四聚磷酸盐和六偏磷酸盐等为磷酸供体，高效再生GTP。将本发明的PPK用于GTP再生，GDP-L-岩藻糖或GDP-甘露糖及其衍生产品合成时(如岩藻糖基乳糖)，显著提高了反应的可持续性，降低反应成本。