Method for creating loofah double haploid through distant hybridization induction

The invention discloses a method for creating double haploids of towel gourds through distant hybridization induction. The method comprises the following steps: applying a brassinolide aqueous solution to female flower ovaries of towel gourds to bloom in the next day; on the second day, pollinating...

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Bibliographische Detailangaben
Hauptverfasser: NI WEICHEN, MENG YING, BI YANFEI, MA XIABIN, FENG CUI, QIAN CHUNYUAN, QIAN CHUNTAO, WAN CHAOQIN, SU XIAOJUN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a method for creating double haploids of towel gourds through distant hybridization induction. The method comprises the following steps: applying a brassinolide aqueous solution to female flower ovaries of towel gourds to bloom in the next day; on the second day, pollinating the male flowers of the wax gourds and the female flowers of the towel gourds to which the brassinolide aqueous solution is applied, applying a thidiazuron aqueous solution to ovaries and pistils of the towel gourds at the same time, and bagging and isolating the female flowers after pollination; applying a thidiazuron solution to the ovary and the fruit stem base 3-6 hours after pollination and 20-26 hours after pollination; after harvesting seed melons, taking relatively full seeds for accelerating germination and sowing to obtain regenerated plants; the regenerated plants are subjected to seedling hardening and transplanting, and then normal cultivation management is conducted. According to the method, thidiazur