Saccharomyces cerevisiae engineering strain for producing p-coumaroyl-coenzyme A as well as construction method and application of saccharomyces cerevisiae engineering strain

The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing p-coumaroyl-coenzyme A. The saccharomyces cerevisiae engineering strain contains a tyrosine ammonia lyase gene TAL as shown in SEQ ID NO.1 and a p-coumaroyl-coenzyme A l...

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Bibliographische Detailangaben
Hauptverfasser:	SONG JIA, WANG MIN, XIA MENGLEI, WANG JIAJIAN, TU LINNA, KONG FANMENG, SUN YIHAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing p-coumaroyl-coenzyme A. The saccharomyces cerevisiae engineering strain contains a tyrosine ammonia lyase gene TAL as shown in SEQ ID NO.1 and a p-coumaroyl-coenzyme A ligase gene 4CL as shown in SEQ ID NO.2. The saccharomyces cerevisiae engineering strain can be used for producing p-coumaroyl-coenzyme A. The invention further discloses a preparation method of the saccharomyces cerevisiae engineering strain. The engineering strain disclosed by the invention can be used for synthesizing p-coumaroyl coenzyme A by taking L-tyrosine as a substrate. The genetically engineered bacterium contains a tyrosine ammonia lyase gene TAL and a p-coumaroyl coenzyme A ligase gene 4CL, the genetically engineered bacterium is stable in genetic character, and the yield of p-coumaroyl coenzyme A is high and can reach 512.36 mg/L. And the fermentation production of the product can be carried out throug