The invention relates to 2apos; preparation method of fucosyllactose and prepared standard substance

The invention discloses a preparation method of 2 '-fucosyllactose and a prepared standard substance, and belongs to the field of dairy product separation.The preparation method comprises the following steps that lactose is removed from pretreated breast milk, then medium and acid breast milk oligosaccharides are separated, and target oligosaccharides in the neutral breast milk oligosaccharides are collected and freeze-dried; the method for removing lactose comprises the following steps: adopting a solid-phase extraction column and/or a sephadex chromatographic column; the separation method of the medium and acid breast milk oligosaccharides comprises the step of separating by adopting a strong anion exchange chromatographic column to obtain the neutral breast milk oligosaccharides. The purity of the prepared 2 '-FL is 84%. According to the invention, breast milk is used as a raw material to prepare the 2 '-FL standard substance, the obtained 2'-FL product can be used as a reference substance for qualitative