Method for detecting bacterial endotoxin content of collagen sponge

The invention provides a method for detecting the bacterial endotoxin content of collagen sponge. The method comprises the following steps: preparing a standard curve and preparing a test sample leaching solution; obtaining the bacterial endotoxin recovery rate of the test sample; and determining the optimal dilution ratio to obtain the bacterial endotoxin content of the collagen sponge. According to the method, the optimal dilution ratio is determined for dilution of the extract liquid of the collagen sponge, interference is eliminated, a simple, reliable and accurate method is established for endotoxin detection, and data support can be provided for safety evaluation of the product. 本发明提供一种胶原蛋白海绵的细菌内毒素含量的检测方法，包括制备标准曲线、制备试验样品浸提液；获取供试样品的细菌内毒素回收率；确定最佳稀释倍数，得到胶原蛋白海绵的细菌内毒素含量。本发明通过对胶原蛋白海绵的浸提液确定最佳稀释倍数稀释，排除干扰，为内毒素检测建立简洁、可靠、准确的方法，可为该产品安全性评价提供数据支持。