Method for extracting sulfonamide antibiotics from plant tissues and application of sulfonamide antibiotics
The invention belongs to the technical field of extraction and detection of residual antibiotics in plants, and particularly relates to a method for extracting sulfonamide antibiotics in plant tissues and application of the sulfonamide antibiotics. According to the method, the plant tissue and the b...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention belongs to the technical field of extraction and detection of residual antibiotics in plants, and particularly relates to a method for extracting sulfonamide antibiotics in plant tissues and application of the sulfonamide antibiotics. According to the method, the plant tissue and the buffer solution with the pH value of 3 are mixed under the dark condition, so that the plant tissue is in an acid environment, the sulfonamide antibiotics in a sample can be well protected, and the natural degradation rate of the sulfonamide antibiotics is reduced; in the extraction link, acidified acetonitrile is used as a unique extracting agent, so that more sulfonamide compounds are dissolved into the acidified acetonitrile from plant tissues; by adding EDTA, metal ions in plant tissues and an extracting solution can be chelated, and the interference effect of ions is reduced. According to the method, the variety and the number of the extracting agents are simplified, and the effect of efficiently extracting the |
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