HPLC (High Performance Liquid Chromatography) detection method for alpha-keto-D-glucuronic acid fermentation liquor

The invention discloses an HPLC (High Performance Liquid Chromatography) detection method for alpha-keto-D-glucuronic acid fermentation liquor, which is characterized in that the alpha-keto-D-glucuronic acid fermentation liquor injected into a specific chromatographic column is eluted by a specific mobile phase, and effective separation of alpha-keto-D-glucuronic acid in the fermentation liquor is realized for the first time under the conditions of specific column temperature and sample injection amount; according to the present invention, the alpha-keto-D-glucuronic acid fermentation broth can be accurately and quantitatively detected, the quality control method of the alpha-keto-D-glucuronic acid fermentation broth is perfected, the quality of the alpha-keto-D-glucuronic acid fermentation broth is effectively controlled, and the fermentation production of the alpha-keto-D-glucuronic acid is ensured. 本发明公开了一种α-酮基-D-葡萄醛酸发酵液的HPLC检测方法，其以特定的流动相洗脱进样于特定色谱柱的α-酮基-D-葡萄醛酸发酵液，在特定的柱温和进样量的条件下，首次实现了对发酵液中α-酮基-D-葡萄醛酸有效分离，使之