Alzheimer's disease protein marker detection module, preparation method and application thereof

The invention belongs to the technical field of biochemical sensing and in-vitro diagnosis, and particularly relates to an Alzheimer's disease protein marker detection module as well as a preparation method and application thereof. The method comprises the following steps: depositing a metal electrode on a silicon insulating substrate; graphene is transferred to the substrate plated with the metal electrode; sequentially dropwise adding a DNA aptamer with one modified end, polyethylene glycol and ethanolamine to the surface of the graphene channel for functionalization; according to the detection module, charged molecules are fixed to the surface of a graphene channel, the conductivity of the surface of the channel is changed, and therefore high-sensitivity and rapid detection of the Alzheimer's disease protein biomarker is achieved; according to the present invention, with the detection method, the requirement of the clinical rapid detection of one or more protein markers of the Alzheimer's disease by using