SNP (Single Nucleotide Polymorphism) site combination, primer combination and identification method for identification of Wumongolian Fengfeng chicken

The invention discloses an SNP (Single Nucleotide Polymorphism) site combination, a primer combination and an identification method for identification of Wumongolian Fengfeng chicken. The site combination comprises a WMF1 site to a WMF6 site. When the site combination provided by the invention is used for identifying the Wumongphoenix chicken resources, as long as one SNP site genotype does not conform to the corresponding genotype of the Wumongphoenix chicken, the individual can be completely excluded from belonging to the Wumongphoenix chicken; and if the six SNP site combinations all conform to the genotype of the WuMongolian Fengfeng chicken, determining that the individual belongs to the Wumongolian Fengfeng chicken. The identification method is simple and convenient to operate, high in accuracy and capable of rapidly identifying UMongolian Fengfeng chicken resources. 本发明公开了一套用于乌蒙凤鸡鉴定的SNP位点组合、引物组合及鉴定方法，所述位点组合包括WMF1位点到WMF6位点。利用本发明中的位点组合进行乌蒙凤鸡资源鉴定时，只要有一个SNP位点基因型不符合乌蒙凤鸡相应的基因型，即可完全排除该个体属于乌蒙凤鸡；6个SNP位点组合全部符合乌蒙