Method for creating diploid seedless watermelon through gene editing ClCKI1

The invention discloses a method for creating diploid seedless watermelons through gene editing ClCKI1, and belongs to the technical field of plant genetic engineering breeding. According to the method for creating the diploid seedless watermelon through gene editing ClCKI1, a CRISPR/Cas9 technology is utilized for the first time, targeted editing is conducted on the watermelon ClCKI1 gene, so that gene functions of the watermelon ClCKI1 gene are lost, female gametes are sterile, selfing pollination fruits are normally expanded, zygotes cannot be formed through double fertilization, seeds are aborted, and only a small amount of white-shell seeds are generated; therefore, diploid seedless watermelons are created. Compared with a wild type material, the diploid seedless watermelon edited plant created by the method disclosed by the invention has no obvious phenotypic change in a vegetative growth stage and a male gamete except for female gamete sterility. The method has the advantages of simplicity, feasibility