Application of inhibitor in increasing tomato fruit size and tomato breeding method

The invention relates to the technical field of plant genetic engineering, in particular to application of an inhibitor in increasing the size of tomato fruits and a tomato breeding method. The embodiment of the invention provides application of an inhibitor in increasing the size of tomato fruits. The inhibitor inhibits expression of an SlMYB64 gene. And/or the inhibitor reduces the activity or content of the encoding protein of the SlMYB64 gene. The embodiment of the invention finds that the SlMYB64 gene is an important factor for regulating and controlling the size of the tomato fruit, the gene is mutated by utilizing a CRISPR-Cas9 technology through an experiment, and a result shows that the size of the tomato fruit is obviously increased, so that a candidate gene is provided for changing the size of the tomato fruit. 本申请涉及植物基因工程技术领域，尤其涉及一种抑制剂在提高番茄果实大小中的应用以及番茄育种方法。本申请实施例提供一种抑制剂在提高番茄果实大小中的应用，该抑制剂抑制SlMYB64基因的表达；和/或，该抑制剂降低SlMYB64基因的编码蛋白的活性或含量。本申请实施例发现SlMYB64基因是调控番茄果实大小的一个重要因子，通过实验利用CRISPR-Cas9技术突变该基因，结果显示番茄果