Mutant gene OsLCA1 of rice low-cadmium accumulation mutant lca1 and application of mutant gene OsLCA1
The invention discloses a mutant gene OsLCA1 of a rice low-cadmium accumulation mutant lca1 and application of the mutant gene OsLCA1. According to the mutant gene OsLCA1 of the rice low-cadmium accumulation mutant lca1, a promoter region of a wild OsLCA1 gene is mutated, namely, the 39 < th >...
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Zusammenfassung: | The invention discloses a mutant gene OsLCA1 of a rice low-cadmium accumulation mutant lca1 and application of the mutant gene OsLCA1. According to the mutant gene OsLCA1 of the rice low-cadmium accumulation mutant lca1, a promoter region of a wild OsLCA1 gene is mutated, namely, the 39 < th > nucleotide at the upstream of ATG is mutated into C from A, a promoter nucleotide sequence is as shown in SEQ ID No.1, and the cadmium content in rice plants and grains of the mutant lca1 is correspondingly lower than that in plants and grains of wild rice, as shown in figure 1. The promoter and a coding amino acid sequence regulated and controlled by the promoter are shown as SEQ ID No.2. According to the application of the mutant gene OsLCA1, the rice contains the mutant gene OsLCA1. The cadmium content in grains of the mutant material lca1 obtained by the invention is reduced to about 30%.
本发明公开了一种水稻低镉积累突变体lca1的突变基因OsLCA1及其应用。一种水稻低镉积累突变体lca1的突变基因OsLCA1,野生OsLCA1基因的启动子区域发生突变,即ATG上游第39个核苷酸由A突变为C,启动子核苷酸序列如SEQ ID No.1所示,突 |
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