Nutritional segmentation fed-batch fermentation method of paecilomyces hepialid

The invention provides a paecilomyces hepialid nutrition segmentation fed-batch fermentation method. A fermentation culture medium is divided into two parts, one part serves as a substrate culture medium to be put into a fermentation tank at a time when fermentation begins, and the other part serves as a nutrient supplement part to be fed from fermentation for 12 h to fermentation completion. 0.1%-0.4% of high free nucleotide yeast hydrolysate and 0.3%-0.6% (volume fraction) of organic acid compound liquid are additionally added into the fed-batch culture medium. On one hand, the growth inhibition effect caused by too high substrate concentration is reduced; on the other hand, the optimized fed-batch culture medium provides sufficient nutrition for growth and metabolism of mycelia, and the problems of insufficient vitality and low yield of thalli in the later stage of fermentation are solved. 本发明提供了一种蝙蝠蛾拟青霉营养分割流加发酵的方法。将发酵培养基分割为两部分，一部分作为底物培养基在发酵开始时一次性投入发酵罐中，另一部分培养基作为营养补充部分从发酵12h开始流加至发酵结束，并在流加培养基中另添加0.1％～0.4％高游