Application of SlFPF1 gene in regulation and control of tomato flowering time and plant type
The invention relates to the technical field of biology, in particular to application of an SlFPF1 gene in regulation and control of tomato flowering time and plant type. The sequence of the protein coded by the SlFPF1 gene is as shown in SEQ ID NO: 3. According to the invention, a pHellsgate 8 vect...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to the technical field of biology, in particular to application of an SlFPF1 gene in regulation and control of tomato flowering time and plant type. The sequence of the protein coded by the SlFPF1 gene is as shown in SEQ ID NO: 3. According to the invention, a pHellsgate 8 vector driven by a CaMV 35S promoter is used for over-expression of an SlFPF1 gene, an obtained SlFPF1 over-expression tomato transgenic line has phenotypes of flowering time delay, internode spacing, epicotyl growth and hypocotyl growth, gene editing is carried out on the tomato SlFPF1 gene through a CRISPR/Cas9 gene editing technology, a tomato transgenic line with SlFPF1 function loss is obtained, and the tomato transgenic line with the SlFPF1 function loss is obtained. The tomato SlFPF1 gene has the phenotype of advanced flowering time and shortened internode spacing, so that the tomato SlFPF1 gene is confirmed to be related to the flowering time and plant type of the tomato, and gene resources are provided for cre |
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