Method for directly inducing radix puerariae adventitious roots by using radix puerariae leaves and root hairs and culture medium formula

The invention provides a method for directly inducing radix puerariae adventitious roots by using radix puerariae leaves and root hairs and a culture medium formula. The invention belongs to the technical field of biology, which comprises the following steps: directly performing adventitious root induction by using leaves and root hairs of the root of kudzu vine as explants, and culturing in an MS (Murashige and Skoog) culture medium which contains 0.5-5.0 mg/L of IBA, 20-30 g/L of cane sugar and 7 g/L of agar and has the pH value of 5.5-6.5. Compared with a method in the prior art that calluses are firstly induced by explants and then adventitious roots are induced by the calluses, the method disclosed by the invention has the advantages of rapid adventitious root induction, high repeatability, short period, convenience in material taking, no influence of natural disasters and the like. 本发明提供一种利用葛根叶片、根须直接诱导葛根不定根方法及培养基配方。本发明属于生物技术领域，利用葛根叶片、根须作为外植体直接进行不定根诱导，培养在含有0.5～5.0mg/L IBA、20～30g/L蔗糖，7g/L琼脂，pH为5.5～6.5的MS培