Full-chain PTH full-liquid-phase immunoassay method based on alternating current magnetization response of superparamagnetic nanoparticles

The invention provides a full-chain PTH full-liquid-phase immunoassay method based on alternating current magnetization response of superparamagnetic nanoparticles. The method comprises the following steps: obtaining a PTH detection reagent; the detection reagent is provided with biological functionalized superparamagnetic nanoparticles; measuring the intensity of the initial magnetization response signal of the solution containing the detection reagent by using detection equipment, and recording the measurement result as U0; uniformly mixing the detected reagent and the detection reagent, incubating at a preset temperature, and waiting for the incubation time to reach a set time; measuring the intensity of the magnetization response signal of the mixed solution of the detected reagent and the detection reagent by using the detection equipment again, and recording the measurement result as U1; calculating a magnetization response intensity change rate between the U0 and the fruit U1; obtaining an expression o