Field in-situ collection method for rape root exudates

The invention relates to the technical field of soil and plant science, in particular to a field in-situ collection method for rape root exudates. The method comprises the following steps: S1, selecting a rape sowing position in a test field block, and carrying out root plate beating, S2, placing a...

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Bibliographische Detailangaben
Hauptverfasser: ZHOU YIXIN, WANG TAO, LIU YUANYUAN, ZHANG SHIBAI, DUAN XIANJIE, LI WEI, QIU HONGSHUAN, SHI LEI, ZHOU CAN, JIAO YINENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of soil and plant science, in particular to a field in-situ collection method for rape root exudates. The method comprises the following steps: S1, selecting a rape sowing position in a test field block, and carrying out root plate beating, S2, placing a porous plate in a soil body, S3, sowing: sowing rape seeds at a position which is parallel to the porous plate and is 15cm away from the porous plate, when 4-5 true leaves grow, carrying out final singling on four seedlings at intervals of 7-9cm, S4, collecting root exudates of rhizosphere soil, non-rhizosphere soil and root exudates in situ, and S5, collecting the root exudates. Through in-situ sampling of soil roots, collection of rape root exudates, extraction of acid phosphatase and organic acid and accurate analysis and determination of the content of acid phosphatase and organic acid, the method is more intuitive, accurate, practical and scientific, provides an important means for systematic research of rape