SNP (Single Nucleotide Polymorphism) chip as well as preparation method and application thereof

The invention relates to an SNP (Single Nucleotide Polymorphism) chip and a preparation method and application thereof, the SNP chip comprises a solid-phase carrier modified with two primers, and a cluster of oligonucleotide probes prepared from a plurality of library sequences is fixed on the solid-phase carrier through the primers, the oligonucleotide probe sequentially comprises a P7 sequence, a linker sequence 1, a specific hybridization sequence and a sequence of at least four nucleotides generated after enzyme digestion of the library sequence from the 5'terminal to the 3 'terminal, and the nucleotides are randomly selected from A, T, C and G. The invention also provides a preparation method of the SNP chip and a kit formed by the SNP chip, the SNP chip has high density, can detect more than 20,000,000 SNP sites, has higher flux, can meet the requirement of large-flux detection of SNP variation sites, and reduces the cost through a scale effect. 本发明涉及一种SNP芯片及其制备方法和应用，所述SNP芯片包括其包括修饰有两种引物的固相载体，通过所述引物，在该固相