Production method of maltooligosacharide with high maltotetraose content

The invention relates to the technical field of oligosaccharide preparation, in particular to a production method of maltooligosacharide with high maltotetraose content, which comprises the following steps: step 1, performing size mixing on starch to prepare starch milk, and liquefying the starch milk to obtain starch liquefied liquid; 2, adjusting the pH value of the starch liquefied liquid obtained in the step 1 to 5.0-5.5, adding maltotetraenzyme, debranching enzyme and alpha-amylase, and saccharifying to obtain maltooligosaccharide syrup; step 3, separating the maltooligosaccharide syrup obtained in the step 2 by using a simulated moving bed to obtain maltooligosaccharide feed liquid; and 4, drying the maltooligosaccharide feed liquid obtained in the step 3 to prepare powder. According to the method, the DE value of the starch liquefied liquid is controlled, and a multi-enzyme synergistic saccharification and simulated moving bed separation technology is adopted, so that the content of maltotetraose is in