Gene sequence of neuropeptide CCHamide as well as preparation method and application of gene sequence
The invention belongs to the technical field of gene engineering, and particularly relates to a gene sequence of neuropeptide CCHamide as well as a preparation method and application of the gene sequence. The nucleotide sequence of the neuropeptide CCHamide gene is as shown in SEQ ID NO. 1 (sequence...
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| creator | ZHANG MIN XIA GUOXU WANG JIALE DING TIANBO CHU DONG |
| description | The invention belongs to the technical field of gene engineering, and particularly relates to a gene sequence of neuropeptide CCHamide as well as a preparation method and application of the gene sequence. The nucleotide sequence of the neuropeptide CCHamide gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention also provides dsRNA of the neuropeptide CCHamide gene, and the nucleotide sequence of the dsRNA is as shown in SEQ ID NO. 2. According to the invention, the transient expression vector of the bemisia tabaci MED cryptic species neuropeptide CCHamide gene is constructed to transform tobacco, and the BtCCH2 protein is over-expressed in the tobacco, so that the capability of bemisia tabaci in acquiring and spreading the tomato chlorosis virus can be remarkably reduced. The bemisia tabaci neuropeptide CCHamide gene plays an obvious role in inhibiting bemisia tabaci from acquiring and spreading the tomato chlorosis virus, and the gene plays an important role in intercepting, preventin |
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The nucleotide sequence of the neuropeptide CCHamide gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention also provides dsRNA of the neuropeptide CCHamide gene, and the nucleotide sequence of the dsRNA is as shown in SEQ ID NO. 2. According to the invention, the transient expression vector of the bemisia tabaci MED cryptic species neuropeptide CCHamide gene is constructed to transform tobacco, and the BtCCH2 protein is over-expressed in the tobacco, so that the capability of bemisia tabaci in acquiring and spreading the tomato chlorosis virus can be remarkably reduced. 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The nucleotide sequence of the neuropeptide CCHamide gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention also provides dsRNA of the neuropeptide CCHamide gene, and the nucleotide sequence of the dsRNA is as shown in SEQ ID NO. 2. According to the invention, the transient expression vector of the bemisia tabaci MED cryptic species neuropeptide CCHamide gene is constructed to transform tobacco, and the BtCCH2 protein is over-expressed in the tobacco, so that the capability of bemisia tabaci in acquiring and spreading the tomato chlorosis virus can be remarkably reduced. 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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Gene sequence of neuropeptide CCHamide as well as preparation method and application of gene sequence |
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